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Identification of a New Motif Required for the 3′–5′ Exonuclease Activity of Escherichia coli DNA Polymerase I (Klenow Fragment): THE RRRY MOTIF IS NECESSARY FOR THE BINDING OF SINGLE-STRANDED DNA SUBSTRATE AND THE TEMPLATE STRAND OF THE MISMATCHED DUPLEX
The Klenow fragment of Escherichia coli DNA polymerase I houses catalytic centers for both polymerase and 3′–5′ exonuclease activities that are separated by about 35 Å. Upon the incorporation of a mismatched nucleotide, the primer terminus is transferred from the polymerase site to an exonuclease si...
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| Main Authors: | , , , |
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| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
American Society for Biochemistry and Molecular Biology
2008
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2440594/ https://ncbi.nlm.nih.gov/pubmed/18448432 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M801053200 |
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