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Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA
BACKGROUND: In a traditional electrophoresis mobility shift assay (EMSA) a (32)P-labeled double-stranded DNA oligonucleotide or a restriction fragment bound to a protein is separated from the unbound DNA by polyacrylamide gel electrophoresis (PAGE) in nondenaturing conditions. An extension of this m...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
BioMed Central
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2435560/ https://ncbi.nlm.nih.gov/pubmed/18533036 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-9-272 |
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