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In-line system containing porous polymer monoliths for protein digestion with immobilized pepsin, peptide preconcentration and nano-liquid chromatography separation coupled to electrospray ionization mass spectroscopy

The use of two different monoliths located in capillaries for on-line protein digestion, preconcentration of peptides and their separation has been demonstrated. The first monolith was used as support for covalent immobilization of pepsin. This monolith with well defined porous properties was prepar...

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Detalhes bibliográficos
Main Authors: Geiser, Laurent, Eeltink, Sebastiaan, Svec, Frantisek, Fréchet, Jean M. J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2435401/
https://ncbi.nlm.nih.gov/pubmed/18342870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2008.02.075
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