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Agar plate screening procedure for cyclic adenosine 3',5'-monophosphate and inhibitors of cyclic nucleotide phosphodiesterase.

A procedure is described for the semiquantitative measurement of cyclic adenosine 3',5'-monophosphate (cAMP) and detection of inhibitors of cAMP phosphodiesterase by an agar plate test. The assay organism was an adenyl cyclase-deficient mutant derived from Escherichia coli HfrH. In the presence of a...

詳細記述

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書誌詳細
出版年:Appl Environ Microbiol
主要な著者: Somers, P J, Higgens, C E
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology (ASM) 1977
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242711/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/201216/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.34.5.604-606.1977
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