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96-Well electroporation method for transfection of mammalian central neurons

Manipulating gene expression in primary neurons has been a goal for many scientists for over 20 years. Vertebrate central nervous system neurons are classically difficult to transfect. Most lipid reagents are inefficient and toxic to the cells, and time-consuming methods such as viral infections are...

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Detalhes bibliográficos
Main Authors: Buchser, William J., Pardinas, Jose R., Shi, Yan, Bixby, John L., Lemmon, Vance P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2424126/
https://ncbi.nlm.nih.gov/pubmed/17140120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000112279
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