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In Vitro Mutagenesis of Bacillus subtilis by Using a Modified Tn7 Transposon with an Outward-Facing Inducible Promoter

A Tn7 donor plasmid, pTn7SX, was constructed for use with the model gram-positive bacterium Bacillus subtilis. This new mini-Tn7, mTn7SX, contains a spectinomycin resistance cassette and an outward-facing, xylose-inducible promoter, thereby allowing for the regulated expression of genes downstream o...

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Detalhes bibliográficos
Main Authors: Bordi, Christophe, Butcher, Bronwyn G., Shi, Qiaojuan, Hachmann, Anna-Barbara, Peters, Joseph E., Helmann, John D.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2423030/
https://ncbi.nlm.nih.gov/pubmed/18408063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00476-08
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