Dual-Substrate Plate Diffusion Assay for Proteases
A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands. The diameter of the zones was linearly proportional to the log of the enzyme activity applied over a r...
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| Publié dans: | Appl Environ Microbiol |
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| Auteur principal: | |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1983
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242253/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6337548/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.45.1.200-204.1983 |
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