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Dual-Substrate Plate Diffusion Assay for Proteases

A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands. The diameter of the zones was linearly proportional to the log of the enzyme activity applied over a r...

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Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteur principal: Montville, Thomas J.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1983
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242253/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6337548/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.45.1.200-204.1983
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