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Optimal Cultural and Physiological Conditions for Handling Streptomyces rimosus Protoplasts

A general procedure for manipulating protoplasts of three Streptomyces rimosus strains was developed. More than 50% regeneration efficiency was obtained by optimizing the osmotic stabilizer concentrations and modifying the plating procedure. Preparation and regeneration of protoplasts were studied b...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Principais autores: Pigac, Jasenka, Hranueli, Daslav, Smokvina, Tamara, Alačević, Marija
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1982
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC242166/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16346135/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.44.5.1178-1186.1982
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