High Production of Thermostable β-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis

By cloning the β-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times. β-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70°C for 15 min, followed by centrifugation t...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Hirata, Haruhisa, Negoro, Seiji, Okada, Hirosuke
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1985
Soggetti:	Physiology and Biotechnology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC241766/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16346825/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.49.6.1547-1549.1985
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