Transformation of Heat-Treated Clostridium acetobutylicum Protoplasts with pUB110 Plasmid DNA

Heat treatment of Clostridium acetobutylicum SA-1 protoplasts at 55°C for 15 min before transformation resulted in expression in this microorganism of the kanamycin resistance determinant associated with plasmid pUB110. No heat treatment, or heat treatment at 65 or 44°C for various time intervals, r...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Lin, Yun-Long, Blaschek, Hans P.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1984
Schlagworte:	Genetics and Molecular Biology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC241604/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16346641/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.48.4.737-742.1984
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