Cloning of noncultivatable human rotavirus by single primer amplification.
A novel, sequence-independent strategy has been developed for the amplification of full-length cDNA copies of the genes of double-stranded RNA (dsRNA) viruses. Using human (Bristol) group C rotavirus as an example, a single amino-linked modified oligonucleotide (primer 1) was ligated to either end o...
Gespeichert in:
| Veröffentlicht in: | J Virol |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1992
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC240952/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1371174/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.66.3.1817-1822.1992 |
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