Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase.

Encephalomyocarditis (EMC) virus RNA-dependent RNA polymerase was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST), which allowed easy purification of the fusion protein by affinity chromatography on immobilized glutathione. Inclusion of a thrombin cleavage site...

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Detalhes bibliográficos

Publicado no:	J Virol
Principais autores:	Sankar, S, Porter, A G
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC240947/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1851868/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.6.2993-3000.1991
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