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Site-directed, virus-free, and inducible RNAi in embryonic stem cells
RNAi is a powerful tool for interrogating gene function in ES cells. Combining the high penetrance of a microRNA-embedded shRNA (shRNA-mir) cassette with a locus-defined, inducible expression strategy, we developed a system for RNAi in mouse ES cells. An shRNA-mir cassette is targeted near the const...
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| Principais autores: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2409230/ https://ncbi.nlm.nih.gov/pubmed/18093939 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0710565105 |
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