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Site-directed, virus-free, and inducible RNAi in embryonic stem cells

RNAi is a powerful tool for interrogating gene function in ES cells. Combining the high penetrance of a microRNA-embedded shRNA (shRNA-mir) cassette with a locus-defined, inducible expression strategy, we developed a system for RNAi in mouse ES cells. An shRNA-mir cassette is targeted near the const...

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Bibliografische gegevens
Hoofdauteurs: Wang, Jianlong, Theunissen, Thorold W., Orkin, Stuart H.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 2007
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2409230/
https://ncbi.nlm.nih.gov/pubmed/18093939
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0710565105
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