High-efficiency formation of influenza virus transfectants.

cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 10(5) viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (10(6) cells) for RNP transfection. In addition to genes...

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Bibliographic Details
Published in:J Virol
Main Authors: Enami, M, Palese, P
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1991
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC240634/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2016777/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.5.2711-2713.1991
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