Role of DNase in recovery of plasmid DNA from Clostridium perfringens.
Recovery of plasmid DNA from Clostridium perfringens 10543A and 3626B cleared lysates was significantly improved by the addition of 0.2% (vol/vol) diethylpyrocarbonate (DEP) before protoplast disruption in the cleared lysate protocol. Three previously undetected, large-molecular-mass plasmids (45.2,...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1984
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC240358/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6089664/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.48.1.178-181.1984 |
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