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Structure of the herpes simplex virus capsid: effects of extraction with guanidine hydrochloride and partial reconstitution of extracted capsids.

Viral B capsids were purified from cells infected with herpes simplex virus type 1 and extracted in vitro with 2.0 M guanidine hydrochloride (GuHCl). Sodium dodecyl sulfate-polyacrylamide gel analyses demonstrated that extraction resulted in the removal of greater than 95% of capsid proteins VP22a a...

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Détails bibliographiques
Publié dans:J Virol
Auteurs principaux: Newcomb, W W, Brown, J C
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1991
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC239799/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1846187/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.65.2.613-620.1991
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