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I(5)S: Wide-Field Light Microscopy with 100-nm-Scale Resolution in Three Dimensions

A new type of wide-field fluorescence microscopy is described, which produces 100-nm-scale spatial resolution in all three dimensions, by using structured illumination in a microscope that has two opposing objective lenses. Illumination light is split by a grating and a beam splitter into six mutual...

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Detalhes bibliográficos
Main Authors: Shao, Lin, Isaac, Berith, Uzawa, Satoru, Agard, David A., Sedat, John W., Gustafsson, Mats G. L.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2397336/
https://ncbi.nlm.nih.gov/pubmed/18326649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.120352
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