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Differential stabilization of reaction intermediates: specificity checkpoints for M.EcoRI revealed by transient fluorescence and fluorescence lifetime studies

M.EcoRI, a bacterial sequence-specific S-adenosyl-l-methionine-dependent DNA methyltransferase, relies on a complex conformational mechanism to achieve its remarkable specificity, including DNA bending, base flipping and intercalation into the DNA. Using transient fluorescence and fluorescence lifet...

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Detalhes bibliográficos
Main Authors: Youngblood, Ben, Bonnist, Eleanor, Dryden, David T.F., Jones, Anita C., Reich, Norbert O.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2396439/
https://ncbi.nlm.nih.gov/pubmed/18385156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn131
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