Novel Wide-Range Quantitative Nested Real-Time PCR Assay for Mycobacterium tuberculosis DNA: Development and Methodology

Previously, we designed an internally controlled quantitative nested real-time (QNRT) PCR assay for Mycobacterium tuberculosis DNA in order to rapidly diagnose tuberculous meningitis. This technique combined the high sensitivity of nested PCR with the accurate quantification of real-time PCR. In thi...

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Bibliografiske detaljer
Main Authors: Takahashi, Teruyuki, Tamura, Masato, Asami, Yukihiro, Kitamura, Eiko, Saito, Kosuke, Suzuki, Tsukasa, Takahashi, Sachiko Nonaka, Matsumoto, Koichi, Sawada, Shigemasa, Yokoyama, Eise, Takasu, Toshiaki
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2008
Fag:
Mycobacteriology and Aerobic Actinomycetes
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2395078/
https://ncbi.nlm.nih.gov/pubmed/18337390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01200-07
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