Recovery, Purification, and Cloning of High-Molecular-Weight DNA from Soil Microorganisms

We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-sal...

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Bibliografiske detaljer
Main Authors: Liles, Mark R., Williamson, Lynn L., Rodbumrer, Jitsupang, Torsvik, Vigdis, Goodman, Robert M., Handelsman, Jo
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2008
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Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394920/
https://ncbi.nlm.nih.gov/pubmed/18359830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02630-07
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