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Recovery, Purification, and Cloning of High-Molecular-Weight DNA from Soil Microorganisms
We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-sal...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
2008
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2394920/ https://ncbi.nlm.nih.gov/pubmed/18359830 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02630-07 |
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