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Intrinsic Protein Fluorescence Interferes with Detection of Tear Glycoproteins in SDS-Polyacrylamide Gels Using Extrinsic Fluorescent Dyes

Intrinsic protein fluorescence may interfere with the visualization of proteins after SDS-polyacrylamide electrophoresis. In an attempt to analyze tear glycoproteins in gels, we ran tear samples and stained the proteins with a glycoprotein-specific fluorescent dye. The fluorescence detected was not...

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Autors principals: Zhao, Zhenjun, Aliwarga, Yulina, Willcox, Mark DP
Format: Artigo
Idioma:Inglês
Publicat: The Association of Biomolecular Resource Facilities 2007
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2392995/
https://ncbi.nlm.nih.gov/pubmed/18166676
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