High pressure fosters protein refolding from aggregates at high concentrations
High hydrostatic pressures (1–2 kbar), combined with low, nondenaturing concentrations of guanidine hydrochloride (GdmHCl) foster disaggregation and refolding of denatured and aggregated human growth hormone and lysozyme, and β-lactamase inclusion bodies. One hundred percent recovery of properly fol...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC23894/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10557267/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.96.23.13029 |
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