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Cloning and constitutive expression of the N-acetylneuraminate lyase gene of Escherichia coli.
The N-acetylneuraminate (NANA) lyase (EC 4.1.3.3) gene from Escherichia coli was self-cloned in E. coli. Transformants were selected by complementation of a NANA lyase-deficient E. coli strain. One clone was found to produce NANA lyase, and it contained a recombinant plasmid, pNAL1, with a 9.0-kilob...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1986
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC238919/ https://ncbi.nlm.nih.gov/pubmed/3008647 |
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