Rapid, simplified method for production and purification of tetanus toxin.
A rapid, simplified method for production and purification of tetanus toxin from bacterial extracts was described. The extracts were prepared by stirring young cells (ca. 45-h culture) of Clostridium tetani in 1 M NaCl-0.1 M sodium citrate, pH 7.5, overnight at 0 to 4 degrees C. The toxin was purifi...
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| Publicat a: | Appl Environ Microbiol |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1985
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238473/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3890743/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.49.4.939-943.1985 |
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