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Specific cleavage of chromosomal and plasmid DNA strands in gram-positive and gram-negative bacteria can be detected with nucleotide resolution

A sensitive and precise in vitro technique for detecting DNA strand discontinuities produced in vivo has been developed. The procedure, a form of runoff DNA synthesis on molecules released from lysed bacterial cells, mapped precisely the position of cleavage of the plasmid pMV158 leading strand orig...

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Hlavní autoři: Zechner, Ellen L., Prüger, Hermann, Grohmann, Elisabeth, Espinosa, Manuel, Högenauer, Gregor
Médium: Artigo
Jazyk:Inglês
Vydáno: The National Academy of Sciences of the USA 1997
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC23839/
https://ncbi.nlm.nih.gov/pubmed/9207109
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