Detection of replicative integrity in small colonic biopsies using the BrdUrd comet assay

The alkaline single-cell gel electrophoresis or comet assay is a relatively simple method of measuring DNA single-strand breaks and alkali-labile sites in individual cells. Previously, we have used a combination of this with bromodeoxyuridine labelling of DNA and immunolocalisation of the BrdUrd to...

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Dettagli Bibliografici
Autori principali: McGlynn, A P, Wasson, G R, O'Reilly, S, McKelvey-Martin, V J, McNulty, H, Strain, J J, McKerr, G, Mullan, F, Mahmud, N, Scott, J, Weir, D G, Downes, C S
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2003
Soggetti:
Molecular and Cellular Pathology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2377088/
https://ncbi.nlm.nih.gov/pubmed/12644827
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.bjc.6600836
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