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Cloning, expression, purification, crystallization and preliminary X-ray studies of a pyridoxine 5′-­phosphate oxidase from Mycobacterium smegmatis

Pyridoxine 5′-phosphate oxidases (PNPOxs) are known to catalyse the terminal step in pyridoxal 5′-phosphate biosynthesis in a flavin mononucleotide-dependent manner in humans and Escherichia coli. Recent reports of a putative PNPOx from Mycobacterium tuberculosis, Rv1155, suggest that the cofactor o...

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Bibliografiske detaljer
Main Authors: Jackson, Colin J., Taylor, Matthew C., Tattersall, David B., French, Nigel G., Carr, Paul D., Ollis, David L., Russell, Robyn J., Oakeshott, John G.
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2008
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2376410/
https://ncbi.nlm.nih.gov/pubmed/18453720
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108011512
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