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A partially folded intermediate conformation is induced in pectate lyase C by the addition of 8-anilino-1-naphthalenesulfonate (ANS)

Addition of 8-anilino-1-naphthalenesulfonate (ANS) to acid-denatured pectate lyase C (pelC) leads to a large increase in the fluorescence quantum yield near 480 nm. The conventional interpretation of such an observation is that the ANS is binding to a partially folded intermediate such as a molten g...

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Библиографические подробности
Главные авторы: Kamen, Douglas E., Woody, Robert W.
Формат: Artigo
Язык:Inglês
Опубликовано: Cold Spring Harbor Laboratory Press 2001
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374217/
https://ncbi.nlm.nih.gov/pubmed/11567103
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