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Crystallization and preliminary X-ray study of native and selenomethionyl β-1,4-mannanase AaManA from Alicyclobacillus acidocaldarius Tc-12-31
AaManA, a β-1,4-mannanase from the thermoacidophile Alicyclobacillus acidocaldarius Tc-12-31, was expressed in Escherichia coli and purified in a form suitable for X-ray crystallographic analysis. Crystals were obtained using the hanging-drop vapour-diffusion method at 291 K using ammonium dihydroge...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
International Union of Crystallography
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2374155/ https://ncbi.nlm.nih.gov/pubmed/18323611 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108002832 |
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