Distance mapping of protein-binding sites using spin-labeled oligosaccharide ligands

The binding of a nitroxide spin-labeled analog of N-acetyllactosamine to galectin-3, a mammalian lectin of 26 kD size, is studied to map the binding sites of this small oligosaccharide on the protein surface. Perturbation of intensities of cross-peaks in the (15)N heteronuclear single quantum cohere...

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Main Authors: Jain, Nitin U., Venot, Andre, Umemoto, Kimiko, Leffler, Hakon, Prestegard, James H.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2001
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374062/
https://ncbi.nlm.nih.gov/pubmed/11604544
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