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Thymidylate kinase of Mycobacterium tuberculosis: A chimera sharing properties common to eukaryotic and bacterial enzymes

We have overexpressed in Escherichia coli the thymidylate kinase of Mycobacterium tuberculosis (TMPKmt). Biochemical and physico-chemical characterization of TMPKmt revealed distinct structural and catalytic features when compared to its counterpart from yeast (TMPKy) or E. coli (TMPKec). Denaturati...

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Detalhes bibliográficos
Main Authors: Munier-Lehmann, HÉLÈNE, Chaffotte, Alain, Pochet, Sylvie, Labesse, Gilles
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2001
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374024/
https://ncbi.nlm.nih.gov/pubmed/11369858
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