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Overproduction, crystallization and preliminary X-­ray analysis of the putative l-ascorbate-6-phosphate lactonase UlaG from Escherichia coli

UlaG, the putative l-ascorbate-6-phosphate lactonase encoded by the ulaG gene from the utilization of l-ascorbate regulon in Escherichia coli, has been cloned, overexpressed, purified using standard chromatographic techniques and crystallized. Crystals were obtained by sitting-drop vapour diffusion...

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Detalhes bibliográficos
Main Authors: Garces, Fernando, Fernández, Francisco J., Pérez-Luque, Rosa, Aguilar, Juan, Baldomà, Laura, Coll, Miquel, Badía, Josefa, Vega, M. Cristina
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373999/
https://ncbi.nlm.nih.gov/pubmed/18097099
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107065256
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