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Overexpression, purification and crystallization of the tetrameric form of SorC sorbitol operon regulator

The sorbitol operon regulator (SorC) regulates the metabolism of l-sorbose in Klebsiella pneumonia. SorC was overexpressed in Escherichia coli and purified, and crystals were obtained of a tetrameric form. A single crystal showed X-ray diffraction to 3.20 Å. The crystal belongs to space group P2(1)2...

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Detalhes bibliográficos
Main Authors: de Sanctis, Daniele, Rêgo, Ana T., Marçal, David, McVey, Colin E., Carrondo, Maria A., Enguita, Francisco J.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373991/
https://ncbi.nlm.nih.gov/pubmed/18097095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107060897
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