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Heterogeneity in the structural dynamics of Sulfolobus solfataricus β-glycosidase revealed by electron paramagnetic resonance and frequency domain fluorometry
The local and global dynamics of the Sulfolobus solfataricus β-glycosidase were studied by electron spin resonance and time-resolved fluorescence techniques. For electron paramagnetic resonance (EPR) investigations, the protein was covalently modified by the maleimido nitroxide spin label, which is...
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| Главные авторы: | , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Cold Spring Harbor Laboratory Press
2002
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2373732/ https://ncbi.nlm.nih.gov/pubmed/12381837 |
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