Heterogeneity in the structural dynamics of Sulfolobus solfataricus β-glycosidase revealed by electron paramagnetic resonance and frequency domain fluorometry

The local and global dynamics of the Sulfolobus solfataricus β-glycosidase were studied by electron spin resonance and time-resolved fluorescence techniques. For electron paramagnetic resonance (EPR) investigations, the protein was covalently modified by the maleimido nitroxide spin label, which is...

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Hauptverfasser: Lozinsky, Evgenia, Febbraio, Ferdinando, Shames, Alexander I., Likhtenshtein, Gertz I., Bismuto, Ettore, Nucci, Roberto
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2002
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373732/
https://ncbi.nlm.nih.gov/pubmed/12381837
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