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Microwave-enhanced enzyme reaction for protein mapping by mass spectrometry: A new approach to protein digestion in minutes

Accelerated proteolytic cleavage of proteins under controlled microwave irradiation has been achieved. Selective peptide fragmentation by endoproteases trypsin or lysine C led to smaller peptides that were analyzed by matrix-assisted laser desorption ionization (MALDI) or liquid chromatography-elect...

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Detalhes bibliográficos
Main Authors: Pramanik, Birendra N., Mirza, Urooj A., Ing, Yao Hain, Liu, Yan-Hui, Bartner, Peter L., Weber, Patricia C., Bose, Ajay K.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2002
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373716/
https://ncbi.nlm.nih.gov/pubmed/12381849
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