PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori

Genes that are characteristic of only certain strains of a bacterial species can be of great biologic interest. Here we describe a PCR-based subtractive hybridization method for efficiently detecting such DNAs and apply it to the gastric pathogen Helicobacter pylori. Eighteen DNAs specific to a monk...

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Autors principals: Akopyants, Natalia S., Fradkov, Arkady, Diatchenko, Luda, Hill, Jason E., Siebert, Paul D., Lukyanov, Sergey A., Sverdlov, Eugene D., Berg, Douglas E.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 1998
Matèries:
Biological Sciences
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC23726/
https://ncbi.nlm.nih.gov/pubmed/9789049
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