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Isolation and characterization of nonsense mutations in gene 10 of bacteriophage phi 6.

Nonsense mutants of bacteriophage phi 6 were isolated by a procedure that involved directed mutagenesis of a cDNA copy of genomic segment M, transcription of this segment, in vitro packaging into procapsids, and transfection of spheroplasts to form viable mutant phage. Recombinant phi 6 viruses that...

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Detalhes bibliográficos
Main Authors: Johnson, M D, Mindich, L
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC236709/
https://ncbi.nlm.nih.gov/pubmed/8139018
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