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Improved Identification of Membrane Proteins by MALDI-TOF MS/MS Using Vacuum Sublimated Matrix Spots on an Ultraphobic Chip Surface

Integral membrane proteins are notoriously difficult to identify and analyze by mass spectrometry because of their low abundance and limited number of trypsin cleavage sites. Our strategy to address this problem is based on a novel technology for MALDI-MS peptide sample preparation that increases th...

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Detalhes bibliográficos
Main Authors: Poetsch, Ansgar, Schlüsener, Daniela, Florizone, Christine, Eltis, Lindsay, Menzel, Christoph, Rögner, Matthias, Steinert, Kerstin, Roth, Udo
Formato: Artigo
Idioma:Inglês
Publicado em: The Association of Biomolecular Resource Facilities 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2361163/
https://ncbi.nlm.nih.gov/pubmed/19137096
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