Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis

A better understanding of Mycobacterium tuberculosis virulence mechanisms is highly dependent on the design of efficient mutagenesis systems. A system enabling the positive selection of insertional mutants having lost the delivery vector was developed. It uses ts-sacB vectors, which combine the coun...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Pelicic, Vladimir, Jackson, Mary, Reyrat, Jean-Marc, Jacobs, William R., Gicquel, Brigitte, Guilhot, Christophe
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1997
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC23543/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9380741/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.20.10955
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