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Isotope-coded, iodoacetamide-based reagent to determine individual cysteine pK(a) values by MALDI-TOF mass spectrometry
Cysteine reactivity in enzymes is imparted to a large extent by the stabilization of the deprotonated form of the reduced cysteine (i.e. the thiolate) within the active site. While this is likely to be an important chemical attribute of many thiol-based enzymes including cysteine-dependent peroxidas...
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| Main Authors: | , , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2007
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2351891/ https://ncbi.nlm.nih.gov/pubmed/18162165 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.12.004 |
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