Isoenergetic penta- and hexanucleotide microarray probing and chemical mapping provide a secondary structure model for an RNA element orchestrating R2 retrotransposon protein function

LNA (locked nucleic acids, i.e. oligonucleotides with a methyl bridge between the 2′ oxygen and 4′ carbon of ribose) and 2,6-diaminopurine were incorporated into 2′-O-methyl RNA pentamer and hexamer probes to make a microarray that binds unpaired RNA approximately isoenergetically. That is, binding...

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Detalhes bibliográficos
Main Authors: Kierzek, Elzbieta, Kierzek, Ryszard, Moss, Walter N., Christensen, Shawn M., Eickbush, Thomas H., Turner, Douglas H.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2008
Assuntos:
RNA
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2346776/
https://ncbi.nlm.nih.gov/pubmed/18252773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm1085
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