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Protein preparation, crystallization and preliminary X-ray crystallographic analysis of SMU.961 protein from the caries pathogen Streptococcus mutans

The smu.961 gene encodes a putative protein of 183 residues in Streptococcus mutans, a major pathogen in human dental caries. The gene was cloned into expression vector pET28a and expressed in a substantial quantity in Escherichia coli strain BL21 (DE3) with a His tag at its N-terminus. The recombin...

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Bibliografische gegevens
Hoofdauteurs: Gao, Xiong-Zhuo, Li, Lan-Fen, Su, Xiao-Dong, Zhao, XiaoJun, Liang, Yu-He
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: International Union of Crystallography 2007
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2339730/
https://ncbi.nlm.nih.gov/pubmed/17909295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107043242
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