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Expression, purification, crystallization and preliminary crystallographic analysis of the mouse transcription factor MafB in complex with its DNA-recognition motif Cmare

The MafB transcription factor (residues 211–305) has been overexpressed in and purified from Escherichia coli. A protein–DNA complex between the MafB homodimer and the 21 bp Maf-recognition sequence known as Cmare has been successfully reconstituted in vitro and subsequently crystallized. The diffra...

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Detalhes bibliográficos
Main Authors: Textor, Larissa C., Wilmanns, Matthias, Holton, Simon J.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335170/
https://ncbi.nlm.nih.gov/pubmed/17671361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107031260
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