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Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas fluorescens AlgK

AlgK is an outer-membrane lipoprotein involved in the biosynthesis of alginate in Pseudomonads and Azotobacter vinelandii. A recombinant form of Pseudomonas fluorescens AlgK with a C-terminal polyhistidine affinity tag has been expressed and purified from the periplasm of Escherichia coli cells and...

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Autori principali: Keiski, Carrie-Lynn, Yip, Patrick, Robinson, Howard, Burrows, Lori L., Howell, P. Lynne
Natura: Artigo
Lingua:Inglês
Pubblicazione: International Union of Crystallography 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335008/
https://ncbi.nlm.nih.gov/pubmed/17565185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107016880
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