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Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas fluorescens AlgK
AlgK is an outer-membrane lipoprotein involved in the biosynthesis of alginate in Pseudomonads and Azotobacter vinelandii. A recombinant form of Pseudomonas fluorescens AlgK with a C-terminal polyhistidine affinity tag has been expressed and purified from the periplasm of Escherichia coli cells and...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
International Union of Crystallography
2007
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2335008/ https://ncbi.nlm.nih.gov/pubmed/17565185 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107016880 |
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