Purification, quaternary structure, composition, and properties of D-ribulose-1,5-bisphosphate carboxylase from Thiobacillus intermedius.

D-Ribulose-1,5-bisphosphate (RuBP) carboxylase has been purified from glutamate-CO2-S2O3(2)-grown Thiobacillus intermedius by pelleting the enzyme from the high-speed supernatant and by intermediary crystallization followed by sedimentation into a discontinuous 0.2 to 0.8 M sucrose gradient. The enz...

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Bibliographic Details
Main Authors: Purohit, K, McFadden, B A, Cohen, A L
Format: Artigo
Language:Inglês
Published: 1976
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC233084/
https://ncbi.nlm.nih.gov/pubmed/819423
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