Purification, crystallization and preliminary crystallographic analysis of a GTP-binding protein from the hyperthermophilic archaeon Sulfolobus solfataricus

A predicted GTP-binding protein from the hyperthermophilic archaeon Sulfolobus solfataricus, termed SsGBP, has been cloned and overexpressed in Escherichia coli. The purified protein was crystallized using the hanging-drop vapour-diffusion technique in the presence of 0.05 M cadmium sulfate and 0.8 ...

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Detalhes bibliográficos
Main Authors: Wu, Hao, Sun, Lei, Brouns, Stan J. J., Fu, Sheng, Akerboom, Jasper, Li, Xuemei, van der Oost, John
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330175/
https://ncbi.nlm.nih.gov/pubmed/17329823
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107008500
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