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Crystallization and X-ray diffraction analysis of the β-ketoacyl-acyl carrier protein reductase FabG from Aquifex aeolicus VF5

The gene product of fabG from Aquifex aeolicus has been heterologously expressed in Escherichia coli. Purification of the protein took place using anion-exchange and size-exclusion chromatography and the protein was then crystallized. Diffraction data were collected to a maximum resolution of 1.8 Å...

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Detalhes bibliográficos
Main Authors: Mao, Qilong, Duax, William L., Umland, Timothy C.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330128/
https://ncbi.nlm.nih.gov/pubmed/17277451
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107000103
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