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A rapid and simple method for extracting human immunodeficiency virus type 1 RNA from plasma: enhanced sensitivity.

We present here a rapid and simple technique for processing human immunodeficiency virus (HIV)-infected plasma by high-speed centrifugation. HIV type 1 virions are pelleted from up to 1 ml of plasma, gently lysed with a nonionic detergent, and directly amplified. The procedure has few manipulations...

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Autors principals: Mulder, J, Resnick, R, Saget, B, Scheibel, S, Herman, S, Payne, H, Harrigan, R, Kwok, S
Format: Artigo
Idioma:Inglês
Publicat: 1997
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC232748/
https://ncbi.nlm.nih.gov/pubmed/9114426
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