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Mutagenesis of hydroxylamine oxidoreductase in Nitrosomonas europaea by transformation and recombination.

Mutagenesis of Nitrosomonas europaea was achieved by electroporation and recombination. To demonstrate this, an aminoglycoside 3'-phosphotransferase (kan) gene was specifically inserted into each of the three gene copies of hao individually. Southern hybridizations and PCR analysis showed the incorp...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Hommes, N G, Sayavedra-Soto, L A, Arp, D J
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC232626/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8682770/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.13.3710-3714.1996
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